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Fig. 2 | BMC Developmental Biology

Fig. 2

From: Generation and identification of a conditional knockout allele for the PSMD11 gene in mice

Fig. 2

Generation of mice with floxed allele of PSMD11. a PCR amplification of region 1 and 2 with primer A1 and A2, or B1 and B2 to identify appropriately targeted embryonic stem cells. Size of the resulting PCR products was 6 kb (MT) for primer A1 and A2, and 310 bp (MT,) and 240 bp (WT) for primer B1 and B2 respectively. b reconfirmation of targeted embryonic stem cell clones by southern blotting. Nde I and Hind III digested DNA from three individual clones that had passed all controls was electrophoretically separated on a 0.5% agarose gel, after transfer to a nylon membrane, a probe targeted against the Neo cassette was hybridized with the digested DNA, which showed two bands at 10.1 kb, and 8.3 kb respectively. c Genotyping of mice with genomic DNA from tail biopsies. Primers F2 and R2 were used to distinguish the floxed allele (PSMD11flx, 463 bp) from the wild-type allele (WT, 335 bp)

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