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Fig. 4 | BMC Developmental Biology

Fig. 4

From: Bivalves are NO different: nitric oxide as negative regulator of metamorphosis in the Pacific oyster, Crassostrea gigas

Fig. 4

Nitric oxide (NO) detection in competent Pacific oyster larvae and spat using the NO-specific fluorescent indicator DAF-FM diacetate. Fluorescent and bright-field images of fluorescent NO signals in competent larvae swimming a left side view, b anterior view on foot, c ventral view on velum, d ventral view on velum with visible apical sensory organ; in larvae with predominant foot crawling on the bottom e left side view after AGH exposure, f anterior view and g left side view after MK-801 exposure; h larvae displaying organ contraction behaviour signalling metamorphosis after EPI exposure; j spat 24 hpe to EPI; control animals without DAF-FM diacetate treatment k in competent larvae without treatment and l spat hpe to EPI. aso: apical sensory organ, a.sh: adult shell growth, ci: cilia of the velum, f: foot, g: gills, gr: gill rudiments, l.sh: larval shell growth, ve: velum, ve.r: rim of velum. Scale bar: 100 μm

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BMC Developmental Biology

ISSN: 1471-213X

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