Fig. 3
Gene expression of CgNOS in Pacific oysters at different larval stages, larvae exposed to different metamorphosis inducers and spat 24 h after metamorphosis induction as well as metamorphosis percentages (%) in Pacific oyster larvae after exposure to inducers. A & C) C. gigas larvae, 18 dpf (d) in January 2018 experiments, exposed to epinephrine (EPI) and MK-801 (MK) at 10− 4 M, L-DOPA at 10− 5 M and ifenprodil (IP) at 10− 6 M and sampled at 4 hpe and 6 hpe. B & D) C. gigas larvae, 17 dpf or 18 dpf for ODQ in March 2019 experiments, exposed to EPI, MK-801, SMIS and 7-NI at 10− 4 M, AGH at 10− 3 M and to ODQ at 5 × 10− 5 M and sampled 3 hpe and 6 hpe. S: spat, C: control spat. Error bars represent standard error. Different lower-case letters represent significant differences with p < 0.05; *: significance not calculated due to differences in sampling days