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Fig. 3 | BMC Developmental Biology

Fig. 3

From: Early shell field morphogenesis of a patellogastropod mollusk predominantly relies on cell movement and F-actin dynamics

Fig. 3

Phalloidin staining showing the dynamics of F-actin during shell field morphogenesis. Distributions of F-actin (phalloidin staining), nucleus (DAPI staining), and merged images at 6–8 hpf are shown in a-c, f-h and k-m, respectively. All panels are lateral views with dorsal to the right and anterior on the top (CLSM optical sections). d, i and n are magnified images of the enclosed regions in a, f and k, respectively; and e, j and o are corresponding schematic diagrams. In the diagrams, the contacts between ectodermal (Ec) and meso/endodermal (Me/En) tissues are indicated by green lines. In j and o, the typical shapes of a central cell (arrows) and a peripheral cells (arrowheads) in the shell field are shown. The red dashed line in o (highlighted by the double arrow) indicates the evident aggregation of F-actin that was located in the posterior part of the embryo and separated ectodermal cells

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