Fig. 5

Summary of genes coding for ion-transport mechanisms and gap-junction subunits showing effects in RNAi-knockdown. a Scheme of a S10B-follicle: somatic cells (turquoise), germline cells (beige). For soma knockdown of relevant genes of ion-transport mechanisms and gap-junction subunits, the tj-Gal4 driver was used, whereas for germline knockdown, the mat-tub-Gal4 and MTD-Gal4 drivers were used. b Out of all performed RNAi-knockdowns (left column: soma knockdown via tj-Gal4; middle and right columns: germline knockdown via mat-tub-Gal4 and MTD-Gal4, respectively), the knockdowns of rpk (first line), inx1 (second line), inx3 (third line; left: size-reduced ovaries with single follicles, middle and right: no effects), and vha55 (fourth line; right: no effects) resulted in striking effects on ovary morphology (DAPI) and/or oogenesis (scale bars represent 100 μm). While soma knockdown of vha55 led to degenerating follicles from S10A onward, germline knockdown of inx1, vha55 and rpk resulted in NC rests and excess of FC around micropyle. The strongest effects were observed for rpk: Reduced rpk-transcript levels in the FE resulted in loss of ovaries, whereas reduced levels in the germline led to size-reduced paired or single ovaries showing ovarioles, but no follicles. c-e Germline driver-directed expression of myrGFP in plasma membranes of NC and Ooc (c: mat-tub-Gal4-GeneSwitch, d mat-tub-Gal4, e MTD-Gal4). myrGFP has an N-terminal myristoylation sequence directing GFP to plasma membranes under UASp-control. For soma driver-directed expression of GFP, see Fig. 1a