Fig. 4
Influences of inhibitors of ion-transport mechanisms on pHi in the FE. a WFM-images of typical experiments showing pHluorin-Moesin-expressing S10B-follicles after incubation in either the respective inhibitor or the control solution (DMSO or ethanol; scale bars represent 200 μm); insets show enlarged examples of representative follicles in pseudocolour (arrows point to FE; scale bars represent 50 μm). Relative alkalisation is indicated by stronger (bright/white), relative acidification by weaker (dark/blue) fluorescence intensities. The experiments were repeated at least four times. b While amiloride caused a slight decrease in fluorescence intensity (acidification), concanamycin A, 9-anthroic acid, furosemide and glibenclamide led to significantly increasing fluorescence intensities (alkalisation). Verapamil showed no effect on pHi. To consider the variability between experiments, mean intensity ratios of the experimental and control groups (inhibited/control) of n = 4 experiments for each inhibitor were calculated. Mean values, shown with their standard deviation, were compared using a one-sample t-test (* p < 0.05; ** p < 0.01). The strongest effects on pHi were obtained with concanamycin A, 9-anthroic acid, furosemide and glibenclamide, respectively