Skip to main content
Fig. 2 | BMC Developmental Biology

Fig. 2

From: Analysing bioelectrical phenomena in the Drosophila ovary with genetic tools: tissue-specific expression of sensors for membrane potential and intracellular pH, and RNAi-knockdown of mechanisms involved in ion exchange

Fig. 2

Genetically-encoded Vmem- and pHi-sensors reveal changes of bioelectrical properties resulting from the inhibition of ion-transport mechasms. a Schematic overview of the analysed ion-transport mechanisms and their specific inhibitors (in brackets). b All inhibitors led to significant changes of Vmem and/or pHi in the FE. While inhibition of NHEs and Na+-channels or L-type Ca2+-channels resulted in relative hyperpolarisation, inhibition of V-ATPases, Cl-channels, Na+/K+−/2Cl-cotransporters or ATP-sensitive K+-channels resulted in relative depolarisation. Concerning pHi, inhibition of V-ATPases, Cl-channels, Na+/K+/2Cl-cotransporters or ATP-sensitive K+-channels resulted in relative alkalisation, whereas inhibition of NHEs and Na+-channels caused relative acidification. The inhibition of L-type Ca2+-channels had no significant effect on pHi. Normalised values of 15 < n < 21 S10B-follicles were averaged (relative intensity). Mean values, shown with their standard deviation, were compared using an unpaired t-test (* p < 0.05; *** p < 0.001)

Back to article page