Fig. 2

Genetically-encoded V_mem- and pH_i-sensors reveal changes of bioelectrical properties resulting from the inhibition of ion-transport mechasms. a Schematic overview of the analysed ion-transport mechanisms and their specific inhibitors (in brackets). b All inhibitors led to significant changes of V_mem and/or pH_i in the FE. While inhibition of NHEs and Na⁺-channels or L-type Ca²⁺-channels resulted in relative hyperpolarisation, inhibition of V-ATPases, Cl⁻-channels, Na⁺/K⁺⁻/2Cl⁻-cotransporters or ATP-sensitive K⁺-channels resulted in relative depolarisation. Concerning pH_i, inhibition of V-ATPases, Cl⁻-channels, Na⁺/K⁺/2Cl⁻-cotransporters or ATP-sensitive K⁺-channels resulted in relative alkalisation, whereas inhibition of NHEs and Na⁺-channels caused relative acidification. The inhibition of L-type Ca²⁺-channels had no significant effect on pH_i. Normalised values of 15 < n < 21 S10B-follicles were averaged (relative intensity). Mean values, shown with their standard deviation, were compared using an unpaired t-test (* p < 0.05; *** p < 0.001)