Skip to main content
Fig. 1 | BMC Developmental Biology

Fig. 1

From: Analysing bioelectrical phenomena in the Drosophila ovary with genetic tools: tissue-specific expression of sensors for membrane potential and intracellular pH, and RNAi-knockdown of mechanisms involved in ion exchange

Fig. 1

Analysis of Vmem and pHi using the genetically-encoded fluorescent Vmem- and pHi-sensors ArcLight and pHluorin-Moesin, respectively. a Uniform expression of GFP in the FE using the soma-driver tj-Gal4 (control); WFM-images of typical tj-Gal4 > UAST-GFP follicles of vitellogenic stages S8-S12 (scale bars represent 50 μm). b, c Pseudocolour images of follicles expressing ArcLight (b) or pHluorin-Moesin (c) in the FE; median optical sections (SIM) of typical follicles of S8-S12 (scale bars represent 50 μm). d Analysis of fluorescence intensities in the FE (“mean grey value”; area marked in yellow); examples of four follicles of S10B (and one of S11) expressing pHluorin-Moesin (WFM-image; scale bar represents 200 μm). e Both the Vmem-sensor ArcLight and the pHi-sensor pHluorin-Moesin use the chromophore pHluorin which responds, in deprotonated state, to blue-light excitation (blue arrow) with the emission of green light (green arrow). The exact mechanism of ArcLight is not known, but is believed to involve voltage-dependent dimerisation leading to protonation of the chromophore (VSD, voltage-sensing domain; scheme inspired by [45]). In cells expressing the respective sensor, relative depolarisation or relative acidification is indicated by weaker fluorescence intensities, whereas relative hyperpolarisation or relative alkalisation is indicated by stronger fluorescence intensities

Back to article page

BMC Developmental Biology

ISSN: 1471-213X

Contact us