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Fig. 3 | BMC Developmental Biology

Fig. 3

From: Multiple functions of the scaffold protein Discs large 5 in the control of growth, cell polarity and cell adhesion in Drosophila melanogaster

Fig. 3

Hippo modulation is not sufficient to explain Dlg5 impact on growth. a control wing and bykiGD40497 RNAi expressing wing with Nubbin:Gal4. c, d, e) quantification of c wing aera) d cell number and e cell size for the indicated genotypes (control n = 9, Dlg5 RNAi n = 16, yki RNAi n = 11). f mutant follicle cell clone for ykiB5, marked by the absence of GFP at stage 9 and stained for Cora. g Quantification of follicle cell size in yki clones compared to wildtype (WT) or yki heterozygous surrounding cells on stage 9 follicles (n = 10). h-k) staining for (h) S6K, i phospho-S6 (pS6) j Phospho-AKT (pAKT) and k Myc in Dlg5ex13 mutant clones in follicle. l Relative quantification of fluorescence for the indicated markers in Dlg5ex13 clones compared to WT surroundings cells (n > 4). m dMyc staining in follicle cell mutant clones for ykiB5.o dMyc staining in wing disc mutant clones for Dlg5ex13. For all pictures scale bar is 10 μm. (** p-value < 0.01, **** p p-value < 0.0001)

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