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Fig. 4 | BMC Developmental Biology

Fig. 4

From: Wingless and Archipelago, a fly E3 ubiquitin ligase and a homolog of human tumor suppressor FBW7, show an antagonistic relationship in wing development

Fig. 4

Knockdown of ago enhances canonical Wg signaling by increasing the level of Arm. a Lethality caused by overexpression of ArmS10 is suppressed by Ago. Only female progeny from crosses between nub-Gal4 females and w1118, UAS-armS10, UAS-armS10UAS-ago RNAi or UAS-armS10UAS-ago males were counted (n > 100 for each group) for survival rate since UAS-ArmS10 transgene is present in the first chromosome. The control cross yielded 45% female and 54% male of nub-Gal4/+. *p < 0.05, **p < 0.01 and ***p < 0.001. b Level of Arm is increased by knockdown of Ago and decreased by overexpression of Ago in cell extracts from larvae expressing GFP, ago RNAi and myc-ago. α-Tubulin as a control. c Knockdown of Ago enhances Wg signaling activity. S2R+ cells were transfected with ago dsRNA or myc-ago, and Wg activity was measured. Results from triplicate experiments are presented (***p < 0.001)

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