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Fig. 3 | BMC Developmental Biology

Fig. 3

From: Wingless and Archipelago, a fly E3 ubiquitin ligase and a homolog of human tumor suppressor FBW7, show an antagonistic relationship in wing development

Fig. 3

ago and wg show an antagonistic relationship. a-d Loss of wing margin by knockdown of wg is rescued by knockdown of ago. C96-Gal4 was used to drive wg RNAi (b), ago RNAi (c) or together (d) at 25 °C. C96-Gal4/+ wing as a control (a). e Lethal phenotype by knockdown of wg is rescued by knockdown of ago. That is, ci-Gal4/CyO females and w1118, UAS-ago RNAi, UAS-wg RNAi or UAS- wg RNAi ago RNAi males were crossed, and their progeny with ci-Gal4, but not CyO, were counted (n > 50 for each group). Because the control cross between ci-Gal4/CyO females and w1118 males yielded 40% ci-Gal4/+ and 60% +/CyO progeny, expected factor 0.67 (40/60), was considered in calculating survival rates of progeny in experimental groups. *p < 0.05. f-j Loss of shafts in chemosensory bristles by knockdown of ago is rescued by knockdown of wg. neur-Gal4/+ control (n = 19, f), neur > ago RNAi (n = 13, g), neur > ago RNAi wg RNAi (n = 8, h) and neur > wg RNAi (n = 16, i) wings. Quantitative analysis of these bristles is presented in (j) (****p < 0.0001). Flies were cultured at 18 °C

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BMC Developmental Biology

ISSN: 1471-213X

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