Fig. 3

The decrease in neuronal number in the neocortex of Efnb2 cKO^Nes is transient. a. Transverse sections of the CP of the neocortex of control and Efnb2^lox/lox; Nes-Cre embryos at different developmental stages (indicated) were immunostained for Tbr1 (red) and Satb2 (green). b. Measurement of CP thickness in control (n = 3 or 4) and Efnb2^lox/lox; Nes-Cre (n = 3 or 4) embryos at all stages. c. Quantification of the number of neurons in the CP of the neocortex at all stages. The graph represents the number of Tbr1+ and Satb2+ neurons in control and Efnb2^lox/lox; Nes-Cre embryos. d. Mean total neuron numbers at each developmental stages of both genotypes were plotted on a curve graph. e. Mean numbers of Tbr1+ neurons at each developmental stage were plotted on a curve graph. f. Mean numbers of Satb2+ neurons at each developmental stage were plotted on a curve graph. Data on graphs are reported as mean ± SEM, unpaired t-test and 1-way ANOVA with Bonferroni’s multiple comparison test (*P < 0.05; ***P < 0.001). CP: cortical plate. Scale bars: 50 μm