Fig. 2
From: Ephrin-B2 paces neuronal production in the developing neocortex
Loss of ephrin-B2 in progenitors impairs neuronal production. a. Transverse sections of the CP of the dorsal neocortex of E16.5 control and Efnb2lox/lox; Nes-Cre embryos were immunostained for Tbr1 (red), Satb2 (green) and DAPI. b. Measurement of CP thickness in control (n = 3) and Efnb2lox/lox; Nes-Cre (n = 5) embryos. The data is the average of lateral, dorsal, medial measurements. c. Quantification of the number of neurons in the CP of the neocortex. The graph represents total neuron numbers in control (n = 3) and Efnb2lox/lox; Nes-Cre (n = 5) embryos. The data is the average of lateral, dorsal, medial counts. d. Quantification of the number of Tbr1+ neurons in medial, dorsal and lateral regions of the CP of the neocortex in control (n = 3) and Efnb2lox/lox; Nes-Cre (n = 5) E16.5 embryos. e. Quantification of the number of Satb2+ neurons in medial, dorsal and lateral regions of the CP of the neocortex in control (n = 3) and Efnb2lox/lox; Nes-Cre (n = 5) E16.5 embryos. f. Quantification of the number of Tbr1-Satb2- neurons in medial, dorsal and lateral regions of the CP of the neocortex in control (n = 3) and Efnb2lox/lox; Nes-Cre (n = 5) E16.5 embryos. g. Transverse sections of the CP of the neocortex of E16.5 control and Efnb2lox/lox; Nex-Cre embryos were immunostained for Tbr1 (red) and Satb2 (green). h. Measurement of CP thickness in control (n = 5) and Efnb2lox/lox; Nex-Cre (n = 5) embryos. The data is the average of lateral, dorsal, medial measurements. i. Quantification of the number of neurons in the CP of the neocortex. The graph represents total neuron numbers in control (n = 5) and Efnb2lox/lox; Nex-Cre (n = 5) embryos. The data is the average of lateral, dorsal, medial counts. Data are reported as mean ± SEM (*P < 0.05; ***P < 0.001; ****P < 0.0001). CP: cortical plate. Scale bars B-G: 50 μm