Fig. 3

IHC Analysis of Cytoglobin protein: a–f IHC analysis of Cygb protein in the regenerated tissues of house gecko tails; a positive control using a section of mouse small intestine, with the arrowhead pointing to the Cygb protein in the mucosa cells (darker brown), with the rest of the brown staining in the background; b negative control using a sample obtained from house gecko tail tissue that did not undergo autotomy; c the whole regenerated house gecko tail tissue on day 3; d. darker brown staining showing the expression of Cygb in fibroblast-like cells (black arrow), in the polygonal shape (yellow arrow) that characterized a neuronal cell, and in epidermal cells (red arrow), with the rest of the brown staining in the background; e Cygb spread in intracellular of muscle cells; f Cygb spread in the adipose tissue (black arrow) and through the epithelial cells (double arrow), at a magnification of 40 × 10); g box plot of Cygb protein expression based on quantitative analysis using ImmunoRatio from day 1 to day 30, showing significant differences between day 1 and day 3, between day 10 and day 13, and between day 21 and day 25