The functions of ocu-miR-205 in regulating hair follicle development in Rex rabbits

Table 4 Effects of ocu-miR-205 on hair follicle density of Rex rabbits (Count/mm²)

Items	Group				R-MSE	P-value
Items	Control	HBAD-GFP	HBAD-ocu-miR-205-GFP	HBAD-ocu-miR-205-5p-sponge-GFP	R-MSE	P-value
Transfection for 7 days
Hair follicle density	125.57 ± 9.63^bc	144.25 ± 11.47^ba	105.02 ± 5.89^c	163.94 ± 16.68^a	32.7817	0.0085
Primary hair follicle density	10.08 ± 0.85	9.31 ± 0.85	7.80 ± 0.63	9.79 ± 0.77	2.2046	0.1902
Secondary hair follicle density	115.49 ± 9.48^bc	134.93 ± 12.01^ba	97.22 ± 5.68^c	154.15 ± 16.65^a	32.9728	0.0113
Secondary/Primary (S/P) ratio	11.92 ± 1.17	16.02 ± 2.67	12.92 ± 1.06	16.38 ± 2.04	5.2469	0.2536
Transfection for 14 days
Hair follicle density	130.54 ± 12.22^b	136.62 ± 8.51^b	119.30 ± 7.24^b	203.34 ± 14.79^a	31.4005	< 0.0001
Primary hair follicle density	10.86 ± 0.54	9.96 ± 0.50	11.92 ± 0.74	10.11 ± 0.70	1.7739	0.1307
Secondary hair follicle density	119.68 ± 12.03^b	126.66 ± 8.28^b	107.37 ± 7.10^b	213.32 ± 14.62^a	30.9035	0.0001
Secondary/Primary (S/P) ratio	11.08 ± 0.96 ^b	12.82 ± 0.82 ^b	9.20 ± 0.78 ^b	21.62 ± 1.94 ^a	3.4505	< 0.0001
Transfection for 21 days
Hair follicle density	145.43 ± 13.71^b	139.58 ± 16.53^b	126.38 ± 9.30^b	208.69 ± 21.42^a	44.8651	0.0049
Primary hair follicle density	14.30 ± 1.19	12.65 ± 0.80	12.92 ± 0.51	15.29 ± 1.28	2.8084	0.2244
Secondary hair follicle density	131.13 ± 13.21^b	126.94 ± 16.57^b	113.46 ± 9.27^b	193.40 ± 21.31^a	44.4697	0.0060
Secondary/Primary (S/P) ratio	9.45 ± 0.91	10.28 ± 1.40	8.86 ± 0.75	13.28 ± 1.85	3.6808	0.1018

Note: Data shown are mean values ± s. d., and n = 8 per group. In the same row, values with no letter superscripts or same letter superscripts mean no significant difference (P > 0.05), with different letter superscripts mean significant difference (P < 0.05)

ISSN: 1471-213X