Fig. 9

Eyespot responses to two contact materials. a Experimental procedure. After the forewing lift procedure, the proximal side of the prospective eyespot was covered with silicone glassine paper, which inhibits eyespot development. The distal side, including the organizer, was covered with plastic film, which does not inhibit eyespot development in most cases. b Possible results. If silicone glassine paper inhibits organizing activity, an even eyespot will emerge (left). In contrast, if silicone glassine paper inhibits signal propagation, an uneven eyespot will emerge (right). c-j Experimental results. The boundary lines between the two materials are indicated by two opposing red arrows. A treated individual or whole hindwing is shown first (c, e, g, and i), and the treated posterior eyespot is enlarged in the following panels (d, f, h, and j). The proximal side of the treated eyespot is highly compromised in all cases, but the distal side develops almost normally in c-f. In g and h, the distal side of the treated eyespot is also compromised, although less so than the proximal side. In i and j, the distal side of the treated eyespot expands, and the proximal side is compromised