Fig. 6

Quantitative analyses of eyespot changes. a Response profiles of 17 treatment modes. Response results (no change, enlargement, and reduction) vary among treatment modes. Based on the result that was obtained most frequently, the treatments could be classified into 3 groups: no change group, enlargement group, and reduction group. b Eyespot change scores of 17 treatment modes. No change, enlargement, and reduction received scores of 0, 1, and − 1, respectively. The mean and standard error are shown. Treatments with positive mean scores are considered to belong to the enlargement group. Treatments with negative mean scores are considered to belong to the reduction group. Treatments with zero or near-zero mean scores are considered to belong to the no change group. c PCA plot of the 3 treatment modes in two-dimensional space using Factor 1 (eigenvalue 2279; variance ratio = 0.846) and Factor 2 (eigenvalue = 414; variance ratio = 0.154). They are well separated. d PCA plot of 17 treatment modes in two-dimensional space using Factor 1 (eigenvalue 18,659; variance ratio = 0.792) and Factor 2 (eigenvalue = 4891; variance ratio = 0.208). The groups defined by the profiles in A are well reflected. e Eyespot size change ratio of 17 treatment modes. Each treatment mode was compared with no treatment. ***: p < 0.001, **: p < 0.01, *: p < 0.05 (based on raw p-values without Bonferroni or other correction)