Fig. 6

Summary of prominent bioelectrical and cytoskeletal differences between the FE of wt and grk. In early vitellogenic stages, as for example S9 (a), patterns of V_mem (white to blue gradient) and pH_i (white to red gradient) in grk are similar to the respective patterns in wt. White refers to stronger fluorescence intensities, corresponding to relative depolarisation or relative alkalisation, while blue (V_mem) and red (pH_i) refer to weaker fluorescence intensities, corresponding to relative hyperpolarisation (blue) or relative acidification (red). Triangles indicate fluorescence-intensity gradients. In S9, both wt and grk show a-p V_mem-gradients, with mFC being hyperpolarised (blue) in relation to neighbouring cFC and pFC (white). The same holds true for a-p pH_i-gradients, with pFC being the most alkaline FC (white). However, the a-p V_mem-gradient in grk is shallower, since mFC are less hyperpolarised relative to neighbouring FC, and the whole FE is more depolarised compared to wt. In S10B (b), in relation to other FC, grk cFC show both slight hyperpolarisation and slight acidification, while dorsal wt cFC show strong hyperpolarisation as well as strong acidification. In both wt and grk, the BC exhibit relatively depolarised V_mem and relatively acidic pH_i. Concerning cytoskeletal organisation in S9 (a), grk bMF are characterised by transversal alignment (orange dashes) and grk MT by longitudinal alignment (green lines), while wt-typical bMF-condensations (orange asterisks) and cell-autonomously organised MT (green circles) are absent. From S10B onward (b), prominent d-v gradients of both V_mem and pH_i appear in the FE of wt, but not grk. This corresponds with peculiarities in both the bMF- and the MT-organisation: In the wt FE, condensations of bMF (orange asterisks) appear in dorsal cFC, and cell-autonomously organised MT (green circles) appear in mFC and pFC. In contrast, in grk, both condensations of bMF and cell-autonomously organised MT are absent from the whole FE