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Fig. 4 | BMC Developmental Biology

Fig. 4

From: Bioelectrical and cytoskeletal patterns correlate with altered axial polarity in the follicular epithelium of the Drosophila mutant gurken

Fig. 4

Compared to wt, the grk FE exhibits striking cytoskeletal differences. Staining of bMF using fluorescent phalloidin (a-l), and staining of MT using an antibody against acetylated α-tubulin (m-x); tangential optical sections (SIM) of typical S8–12 follicles. a-l Fluorescent phalloidin (bMF) stained wt (a-f) and grk (g-l) follicles. m-x Anti-tubulin (MT) stained wt (m-r) and grk (s-x) follicles (scale bars respresent 20 μm; composed micrographs show different regions of the same follicle, except for (l). The grk FE shows prominent pecularities concerning the bMF-pattern: In contrast to wt, bMF in grk are characterised by strict transversal alignment during all analysed stages (g-l), while wt-typical condensations of bMF in cFC (S9, b) and dorsal cFC (S10B, d) are absent. However, in grk S11, many FC show no bMF (k). In several grk S12 follicles, bMF were almost totally missing (l1), while other follicles (l2) exhibit transversally aligned bMF which are less strictly transcellularly organised than in wt. On the other hand, we observed strict longitudinal MT-alignment in the whole grk FE during all analysed stages (s-x), whereas in the wt FE, longitudinal MT-alignment continuously expands from cFC (S9, n) to pFC (S12, r). For variability of follicles in S9 and S10B, see Additional file: Fig. S2; for numbers of analysed follicles, see Additional file: Table S3

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