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Fig. 9 | BMC Developmental Biology

Fig. 9

From: Electrochemical gradients are involved in regulating cytoskeletal patterns during epithelial morphogenesis in the Drosophila ovary

Fig. 9

The MT-organisation is affected by alterations of both pHi and Vmem. Using inhibitors of ion-transport mechanisms, we modified pHi and/or Vmem as well as the MT-pattern. The results obtained with living αTub84B-GFP follicles (right columns) were similar to those obtained with fixed wild-type follicles using an antibody against acetylated α-tubulin (left columns). Typical follicles of S10b are shown. For summary, see Fig. 10. Scale bars refer to all pictures in the same column. The inhibitor verapamil (voltage-dependent L-type Ca2+-channels), which had the strongest impact on Vmem (strong hyperpolarisation, reduction of the angles of the a-p and the d-v gradient, cf. Figure 2) and led to alkalisation, stabilised the longitudinal alignment of MT. In addition, the MT-bundles appeared to be thicker than in the control (ethanol). On the other hand, the MT of follicles treated with either furosemide (Na+/K+/2Cl-cotransporters; no clear effect on Vmem; strong alkalisation, cf. Figure 2) or glibenclamide (ATP-sensitive K+-channels; moderate hyperpolarisation; strong alkalisation, cf. Figure 2) lost their longitudinal alignment and became partially disintegrated. This coincided with a spherical FC shape, which was especially noticeable in αTub84B-GFP (control DMSO). With 9-anthroic acid (Cl-channels; no clear effect on Vmem; slight alkalisation, cf. Figure 2), this effect was weaker (control ethanol)

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