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Fig. 7 | BMC Developmental Biology

Fig. 7

From: Electrochemical gradients are involved in regulating cytoskeletal patterns during epithelial morphogenesis in the Drosophila ovary

Fig. 7

The bMF-organisation is affected by alterations of both pHi and Vmem. Using inhibitors of ion-transport mechanisms, we modified pHi and/or Vmem as well as the bMF-pattern. The results obtained with living Lifeact-GFP follicles (right columns) were similar to those obtained with fixed wild-type follicles using fluorescent phalloidin (left columns). Typical follicles of S10b are shown. For summary, see Fig. 8. Scale bars refer to all pictures in the same column. The inhibitors glibenclamide (ATP-sensitive K+-channels) or furosemide (Na+/K+/2Cl-cotransporters), which both caused strong alkalisation (cf. Figure 2), resulted in parallel alignment of bMF in all FC (control DMSO). Glibenclamide, which led to (moderate) hyperpolarisation, stabilised the bMF-bundles, while furosemide, which had no clear effect on Vmem, caused partial disintegration of bMF. The inhibitor 9-anthroic acid (Cl-channels), which resulted in slight alkalisation and no clear effect on Vmem (cf. Figure 2), also reduced the frequency of bMF-condensations (control ethanol). On the other hand, bafilomycin (V-ATPases) or amiloride (Na+/H+-exchangers, Na+-channels), both acidifying inhibitors with no strong impact on Vmem (cf. Figure 2), led to an increasing area of bMF-condensation followed by disintegration of bMF in cFC and dorsal mbFC

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