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Fig. 2 | BMC Developmental Biology

Fig. 2

From: Electrochemical gradients are involved in regulating cytoskeletal patterns during epithelial morphogenesis in the Drosophila ovary

Fig. 2

Bioelectrical properties were modified using inhibitors of ion-transport mechanisms (summarised according to [16]). a Schematic drawing of a follicle cell showing the analysed ion-transport mechanisms. Na+/H+-exchangers (NHE) and Na+-channels were blocked with amiloride, V-ATPases with bafilomycin, ATP-sensitive K+-channels with glibenclamide, voltage-dependent L-type Ca2+-channels with verapamil, Cl-channels with 9-anthroic acid and Na+/K+/2Cl-cotransporters with furosemide. Intracellular pH (pHi) and membrane potential (Vmem) were analysed in living follicles using the pH-indicator 5-CFDA,AM (5-carboxyfluorescein diacetate, acetoxymethyl ester) and the potentiometric dye DiBAC4(3) (bis-(1,3-dibutylbarbituric acid) trimethine oxonol). pHi, Vmem or both parameters were affected by each inhibitor [16]. b Schematic summary of the effects of inhibitors on the electrochemical gradients in the columnar FCE during S10b [16]. The antero-posterior (a-p) and dorso-ventral (d-v) pHi- and Vmem-gradients are visualised as colour gradients in the FCE. Triangles symbolise directions of the gradients. Increasing pHi means more alkaline, decreasing Vmem means less hyperpolarised. The effects of inhibitors on the angles of the gradients are represented by width and colour of the triangles. While the strongest effects on pHi-gradients were generated by furosemide or glibenclamide, the strongest effects on Vmem-gradients were generated by verapamil or glibenclamide (bold letters) [16]

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