YWHA (14-3-3) protein isoforms and their interactions with CDC25B phosphatase in mouse oogenesis and oocyte maturation

Table 2 Primers used to detect the presence of each Ywha isoform by RT-PCR

Gene	Forward Primer	Reverse Primer	Product Size
Ywhab	5′-AACGATGTGCTGGAGCTGT-3’	5′-CGGATGCAACTTCAGAAAGA-3’	121 bp
Ywhae	5′-CAGAACTGGACACGCTGAGT-3’	5′-TTCTGCTCTTCACCATCACC-3’	118 bp
Ywhah	5′-CATGAAGGCGGTGACAGA-3’	5′-TAACCCTCCAAGAAGATCGC-3’	110 bp
Ywhag	5′-TCCTTCTTTCCAGCCGATCC-3’	5′-GTTCAGCTCGGTCACGTTCTT-3’	139 bp
Ywhaq	5′-CGGTGGCCTACAAAAACGTG-3’	5′-ACAATTCCAGGACCGTGGTG-3’	168 bp
Ywhaz	5′-CCAGCGACCACCCATTGT-3’	5′-ACGATGACGTCAAACGCTTC-3’	139 bp
Sfn	5′-TGTGGCGAAGACTAGGAGGA-3’	5′-GTCTCGAGAGTAACGCTGGG-3’	134 bp
H2AfZ	5′-TGCAGCTTGCTATACGTGGA-3’	5′-TCCTTTCTTCCCGATCAGCG-3’	110 bp

ISSN: 1471-213X