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Table 2 Primers used to detect the presence of each Ywha isoform by RT-PCR

From: YWHA (14-3-3) protein isoforms and their interactions with CDC25B phosphatase in mouse oogenesis and oocyte maturation

Gene Forward Primer Reverse Primer Product Size
Ywhab 5′-AACGATGTGCTGGAGCTGT-3’ 5′-CGGATGCAACTTCAGAAAGA-3’ 121 bp
Ywhae 5′-CAGAACTGGACACGCTGAGT-3’ 5′-TTCTGCTCTTCACCATCACC-3’ 118 bp
Ywhah 5′-CATGAAGGCGGTGACAGA-3’ 5′-TAACCCTCCAAGAAGATCGC-3’ 110 bp
Ywhag 5′-TCCTTCTTTCCAGCCGATCC-3’ 5′-GTTCAGCTCGGTCACGTTCTT-3’ 139 bp
Ywhaq 5′-CGGTGGCCTACAAAAACGTG-3’ 5′-ACAATTCCAGGACCGTGGTG-3’ 168 bp
Ywhaz 5′-CCAGCGACCACCCATTGT-3’ 5′-ACGATGACGTCAAACGCTTC-3’ 139 bp
Sfn 5′-TGTGGCGAAGACTAGGAGGA-3’ 5′-GTCTCGAGAGTAACGCTGGG-3’ 134 bp
H2AfZ 5′-TGCAGCTTGCTATACGTGGA-3’ 5′-TCCTTTCTTCCCGATCAGCG-3’ 110 bp