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Fig. 8 | BMC Developmental Biology

Fig. 8

From: YWHA (14-3-3) protein isoforms and their interactions with CDC25B phosphatase in mouse oogenesis and oocyte maturation

Fig. 8

In vitro fertilization of eggs from Ywhae knockout females. a For each of five different females with oocyte-specific knockout of Ywhae, the total number of eggs collected and inseminated is indicated in parentheses and the percent of those eggs that proceeded to the two-cell stage is indicated in the bar graph. Two representative examples of fertilization and percent of two cell development of wild-type eggs are shown. In two cases (females 4 and 5), fertilized eggs from Ywhae gene knockout females were continued in culture and developed to the morula and/or blastocyst stage. b Representative images of eggs or two-cell embryos following in vitro fertilization of eggs from oocyte-specific Ywhae knockout females or a wild-type female. c Representative Western blot of cell lysates of eggs collected from wild-type females, lysates of oocyte-specific knockout of Ywhae (conditional knockout or CKO), lysates of eggs collected from the global knockout of Ywhae (GKO), and lysates of two-cell embryos from fertilized wild-type eggs and from fertilized eggs of females with oocyte-specific knockout of Ywhae (conditional knockout or CKO). The blots were probed with antibodies for YWHAE or tubulin (loading control). YWHAE protein was not detected in the knockout eggs nor in the two-cell embryos

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