Fig. 5

Injection of the YWHA-inhibitory peptide and Ywha mRNA antisense morpholinos against mRNA for each Ywha isoform. For each injection condition and the uninjected controls in this series of experiments, the number of cells with GVBD is represented as a percent of the total number of cells studied (indicated at the top the corresponding bar). See the Methods section for experimental methods. a Percent germinal vesicle breakdown (GVBD) in mouse oocytes injected with the YWHA-inhibitory peptide R18, water or uninjected. The data is combined from two repeated experiments. Fisher’s exact test and pairwise comparison of the percentage of GVBD in the control uninjected cells and control water-injected cells revealed no significant difference between those groups, while a significantly greater percentage of GVBD was noted for oocytes injected with R18 compared to control water-injected cells (* in A). b Percent GVBD in oocytes injected with antisense morpholinos against each of the seven mammalian Ywha mRNA isoforms as well as the standard nonsense moprholino that was designed to have no effect. The data is combined from two repeated experiments. A significantly greater percentage of GVBD was noted for oocytes injected with morpholino targeting Ywhah mRNA compared to control non-sense morpholino (* in B)