Fig. 5
From: A modifier in the 129S2/SvPasCrl genome is responsible for the viability of Notch1[12f/12f] mice
qRT-PCR of Notch pathway genes expressed by Notch1[12f/12f] embryos. RNA from E9.5 and E10.5 embryo and control heads (n = 3 of each) was converted to cDNA and tested in replicate qRT-PCR experiments using Actb and Gapdh housekeeping genes. Values for mutants and controls compared to each housekeeping gene were obtained and fold-difference between mutants and controls (taken as 1.0) was determined. The data were plotted using Prism version 8.0. Significance was determined by unpaired Student’s t test, (*) p = 0.07; * p < 0.05; ** p < 0.005