Fig. 6

Effects of PARM1 antisense oligonucleotide GapmeR and heat shock at day 5.5 after fertilization on competence of cleaved embryos to become a blastocyst at day 7.5 after fertilization (Experiment 3). At day 5.5 after fertilization, embryos were subject to a heat shock of 41 °C for 24 h (Heat Shock; hatched bars) or were continued to be cultured at 38.5 °C (i.e., Control; solid bars). All embryos were cultured at 38.5 °C thereafter. Data are least-squares means ± SEM. Heat shock treatment decreased (P = 0.02) the percent of cleaved embryos becoming a blastocyst at day 7.5 after fertilization. There was no no effect of GapmeR treatment or interaction between effects of heat shock and GapmeR treatment on percent of cleaved embryos becoming a blastocyst at day 7.5 after fertilization