Fig. 7

Synergistic effect of insulin and JH II on ovarian development of M. vitrata. a Effect of decapitation and subsequent hormone treatment on oocyte development. Decapitation was performed in teneral female adults soon after emergence (< 12 h). JH II or porcine insulin was injected into decapitated females at 1 μg per female using a microsyringe. ‘CON’ represents females without decapitation treatment. After 5 days at 25 °C, 10 females in each treatment were assessed for oocyte development. Different letters above standard deviation bars indicate significant difference among means at Type I error = 0.05 (LSD test). b Photos showing ovaries from females treated with decapitation or hormone addition compared to untreated (CON) female ovary. Scale bar represents 1 mm