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Fig. 3 | BMC Developmental Biology

Fig. 3

From: Insulin signaling mediates previtellogenic development and enhances juvenile hormone-mediated vitellogenesis in a lepidopteran insect, Maruca vitrata

Fig. 3

Effect of larval nutrients on adult ovarian development of M. vitrata. a Oocyte development according to female age. Ovarioles were separated from different aged females (1–8 days after emergence) to count the number of previtellogenic oocytes (PV), vitellogenic oocytes (VT), and chorionated (CH) oocytes. For each age treatment, 10 females were analyzed. b Influence of pupal weight on ovarian development. Ten females (5 days old after emergence) were randomly collected from three body weight pupal groups and assessed in oocyte development. c Effect of larval diets on oocyte development. A standard artificial diet (‘AD’), three adzuki bean diets in different nutritional amounts, and three cowpea diets in different nutritional amounts were assessed for this analysis. For each diet treatment, 10 females (5 days old after emergence) were randomly selected and their oocyte development was assessed. d Expression levels of Vg and VgR in adults developed from larvae treated with different diets. Expression levels of these two gene were quantified by RT-qPCR in 5 days old females. All treatments were independently replicated three times. β-Actin expression was used as reference gene of RT-qPCR to normalize target gene expression level. Different letters above standard deviation bars indicate significant difference among means at Type I error = 0.05 (LSD test)

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