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Table 3 Effect of t2iGöLIF in bovine embryos

From: A dose-dependent response to MEK inhibition determines hypoblast fate in bovine embryos

Treatments n N Total cells ICM TE ICM:TE NANOG:ICM
DMSO 4 15 261 ± 15a 53 ± 7c 210 ± 20a 0,25 ± 0,05 0,45 ± 0,02b
t2iGö 3 10 282 ± 17a 75 ± 10b 198 ± 19a 0,39 ± 0,08 0,53 ± 0,04a
h-LIF 3 10 281 ± 17a 91 ± 12a 180 ± 18b 0,50 ± 0,08 0,42 ± 0,01b
t2iGö h-LIF 3 9 209 ± 13b 55 ± 8c 142 ± 14c 0,38 ± 0,08 0,59 ± 0,02a
  1. Number of inner cell mass (ICM), trophectoderm (TE) and total cells in embryos cultured under different conditions. “n” is the number of independently IVF runs and “N” is the number of embryos analyzed. Values are mean ± S.E. estimated by a GLMM analysis with Poisson distribution and log as link function (for Total Cells, ICM and TE), and with Binomial distribution with logit as link function for (ICM:TE and NANOG:ICM ratios). Different letters denote significant differences, p < 0.05