Fig. 1

Expression of NANOG, SOX2 and SOX17 in day 2–8 bovine embryos. a Immunofluorescence detection of NANOG, SOX2 and SOX17 in different stage embryos. b Line scan plot of relative intensity of NANOG, SOX2 and SOX17 in ICM cells of day 8 blastocysts. A line was digitally drawn with FIJI software to measure the fluorescence intensity of different cells and those with and without co-expression of the markers. Cells pointed by an arrow were identified in the scan plot (with grey background) to represent arbitrary fluorescence values. Scale bar: 10 μm. c Scatter plot representing number of cells expressing only NANOG, only SOX2, only SOX17 or their co- expressed number of cells in day 8 blastocysts. Data are presented as Median ± IQR