Fig. 7From: An annual cycle of gene regulation in the red-legged salamander mental gland: from hypertrophy to expression of rapidly evolving pheromonesCIRBP titration curve. TAMRA-labeled PMF 3′ UTR 99–368 RNA was titrated with increasing concentrations of rCIRBP/ECFP (black), rCIRBP-RRM/ECFP (blue), and rCIRBP-LCD/ECFP (red), and binding measured by fluorescence quenching. Data were fit to the Hill equation by nonlinear modeling to obtain measures of binding affinity (KD) and cooperativity (n). No significant change was detected for rCIRBP-RRM/ECFP, denoted by a dashed lineBack to article page