Fig. 5From: An annual cycle of gene regulation in the red-legged salamander mental gland: from hypertrophy to expression of rapidly evolving pheromonesCIRBP – PMF 3′ UTR interaction. EMSA using a constant amount of PMF 3′ UTR in vitro RNA (200 ng) with increasing concentrations of rCIRBP/ECFP (μM; RBP), and a protein-only control. Protein fluorescence was detected by ECFP (green), and RNA was stained using Sybr Green II (red)Back to article page