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Fig. 5 | BMC Developmental Biology

Fig. 5

From: Androgen receptor expression is required to ensure development of adult Leydig cells and to prevent development of steroidogenic cells with adrenal characteristics in the mouse testis

Fig. 5

Control of adrenal cell development/differentiation in the testis depends largely on AR expressed in the Leydig cells and peritubular myoid cells. a Testicular expression of Mc2r and Cyp11b1 in mice lacking ARs in different cell types. Transcript levels are measured relative to the external standard (Luc). Data were analysed by single-factor ANOVA or t-test, apart from Cyp11b1 levels in SCARKO, PTM.ARKO, PTM.SCARKO mice which was analysed initially using the Kruskal-Wallis test. The Kruskal-Wallis test showed a significant (P < 0.001) overall difference between groups which were further analysed using single sample t-tests which showed each group was significantly different to control. Different letter superscripts indicate differences (P < 0.05) between groups (analysed by ANOVA) while the presence of an asterisk indicates a significant difference to control (analysed by t-test). For all data, the mean ± SEM of between 7 and 9 animals in each group is shown. b Effect of androgen receptor (AR) knockdown on transcript levels in a Leydig tumor cell (MLTC-1) line. Cells were transfected with vehicle, lentivirus containing control, “scrambled” miRNA or lentivirus containing miRNAs which target the Ar. Data was analysed by 2-factor ANOVA and groups with different letter superscripts are significantly different. Each group shows the mean ± SEM of 3 or 4 individual experiments. Primary raw data are shown in Additional file 1

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