Table 1 Sequences of primers used in the present study
Primer Name F—Forward/R—Reverse | DNA-Sequence 5′-3′ | Annealing Temperature (°C) | Fragment Size (bp) |
|---|---|---|---|
Gene-specific Primer pairs for RACE (GsP) | |||
Gspdnaja1–5′ | 5’ –TTACCGTGTCTTTGGAGGAACTG − 3′ | 61.0 | - |
Gspdnaja1–3′ | 5’–GCAACTCCATAGCCATAACCAG − 3’ | 59.2 | - |
Gspdnajb1–5′ | 5’- GCGACGAGACGCCAACCAACA − 3’ | 68.6 | - |
Gspdnajb1–3’ | 5’- GGGATGTTGGTTGGCGTCTCGTC − 3’ | 69.4 | - |
Primers for RT-qPCR | |||
Dnaja1-F | 5′ –AAAACCCAGCAGAAGGAGACA-3′ | 58.9 | 259 |
Dnaja1-R | 5’ –AGTTCCTCCAAAGACACGGTAAG-3’ | 59.6 | |
Dnajb1-F | 5’-GCGACGAGACGCCAACCAACA-3′ | 68.6 | 151 |
Dnajb1-R | 5′-GGGACGCTCACCGTACAACCACA − 3’ | 68.7 | |
18sRNA primers | |||
18sRNA-R | 5′- TGATTGGGACTGGGGATTGAA-3′ | 59.2 | 232 |
18sRNA-F | 5′- TAGCGACGGGCGGTGTGT-3′ | 62.4 | |