Fig. 2From: Differential roles of insulin like growth factor 1 receptor and insulin receptor during embryonic heart developmentImmunofluorescence detection of activated (phosphorylated) receptors. Sections through the ventricular wall of control and conditional Igf2 mutant embryos at E11.5, E12.5, and E14.5 are shown. Phospho-specific primary antibodies against pIGF1R and pINSR were used as indicated, or no primary antibody was used as a negative control for background. Pairs of images show the phospho-receptor staining in the red channel alone (top of each pair), or merged (lower of each pair) with the signal for a cardiomyocyte marker (see Methods; green channel) and for DNA (DAPI, blue channel). Abbreviations: BW, body wall; V, ventricle. Scale bar: 100 μmBack to article page