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Fig. 2 | BMC Developmental Biology

Fig. 2

From: Differential roles of insulin like growth factor 1 receptor and insulin receptor during embryonic heart development

Fig. 2

Immunofluorescence detection of activated (phosphorylated) receptors. Sections through the ventricular wall of control and conditional Igf2 mutant embryos at E11.5, E12.5, and E14.5 are shown. Phospho-specific primary antibodies against pIGF1R and pINSR were used as indicated, or no primary antibody was used as a negative control for background. Pairs of images show the phospho-receptor staining in the red channel alone (top of each pair), or merged (lower of each pair) with the signal for a cardiomyocyte marker (see Methods; green channel) and for DNA (DAPI, blue channel). Abbreviations: BW, body wall; V, ventricle. Scale bar: 100 μm

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