Table 3 Specific primers used to amplify the transcripts of NKL genes from cDNA of the onychophoran E. rowelli
Gene | Fragment length (in bases) | Direction | Primer sequence | Annealing Temperature (°C) |
|---|---|---|---|---|
NK2.1 | 823 | Forward | TTCTGCATAATAAGGCGGGC | 62 |
Reverse | TTACCACTCCCGTCATTTCC | |||
vax | 543 | Forward | TATCGACTGTCTCCTCTGTCC | 62 |
Reverse | AAAGACGGCCTTCGAAATGGC | |||
Emx | 742 | Forward | CCACGCCTATAGTTCCTAAGC | 62 |
Reverse | TCCTCACCTTGAAGTGTCTCC | |||
Nedx | 795 | Forward | ATGGAAGAAGAGGCCACCAG | 62 |
Reverse | AGGAGGAATGACACCCATAGG | |||
Hhex | 696 | Forward | CATTGGGATGGCTACCTATGG | 62 |
Reverse | CCTGTACTGCTAGCAACAACC | |||
Bari | 411 | Forward | CAAACTACTAAATCAAAGAAACCGAGA | 62 |
Reverse | TTAGTGTTGACACCCAGGTACAAACAT | |||
BarH | 680 | Forward | GGAGGGTTCTAGCAGTAATGC | 58 |
Reverse | GTGTTGGATAGGGCCAATAGG |