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Table 2 Number of female spiders per sample that were used to determine the PtVg4 expression

From: Vitellogenins in the spider Parasteatoda tepidariorum – expression profile and putative hormonal regulation of vitellogenesis

Stage of tested development

Number of spiders per sample

Midgut glands

Ovaries

Penultimate nymphal stage

10

20

Last nymphal stage

5

10

Day of sexual maturity was reached

5

5

Three days after mating

5

5

Day of the first oviposition

3

3

  1. The number of P. tepidariorum females that were used to prepare one sample from the midgut glands and ovaries to determine any fluctuations in the Vg expression at various ages