From: Vitellogenins in the spider Parasteatoda tepidariorum – expression profile and putative hormonal regulation of vitellogenesis
Stage of tested development
Number of spiders per sample
Midgut glands
Ovaries
Penultimate nymphal stage
10
20
Last nymphal stage
5
Day of sexual maturity was reached
Three days after mating
Day of the first oviposition
3