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Fig. 3 | BMC Developmental Biology

Fig. 3

From: Computational fluid dynamic analysis of physical forces playing a role in brain organoid cultures in two different multiplex platforms

Fig. 3

Neurogenesis in the early stages of organoid development. a Top, representative nestin immunostaining of organoids after 10, 14, and 30 days in culture. Bars = 500 μm. Bottom, quantification of intensity of nestin staining normalized with area of organoid for 10, 14 and 30 days in culture for the different conditions. N = 8, 7, 7 and 6, respectively. b Left, representative MAP2 and TBR2 staining of 30-day-old organoids. Bars = 500 μm. Right, quantification of intensity of MAP2 and TBR2 staining. N = 7 organoids for each condition. c Left, representative phospho-histone H3 staining of ventricular-like regions of 30-day-old organoids. Bars = 100 μm. and right, corresponding quantification. N = 4 for each condition. For all staining, organoids were collected from 2 independent experiments. Mean + SD for all quantifications

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