Table 3 Primers used for quantitative RT-PCR
From: Interleukin-6 increases inner cell mass numbers in bovine embryos
Gene | Reference or Genbank accession no. | Primer sequence (5′ - 3′) | Product size (bp) |
|---|---|---|---|
IL6 | NM_173923.2 | Forward: CCAGCCACAAACACTGACCT | 121 |
Reverse: TAGCTCTCAGGCTGAACTGC | |||
IL6R | NM_001110785.3 | Forward: AAGTGCACACCCGTCGTATT | 114 |
Reverse: TCAGATTCAAGGCTGCTGGG | |||
IL6ST | XM_010816769.3 | Forward: GTCTCATGCTCACGGCACTA | 220 |
Reverse: CGCGTCTGATTTGCCAACAA | |||
SDHA | (Goossens et al. 2005) | Forward: GCAGAACCTGATGCTTTGTG | 185 |
Reverse: CGTAGGAGAGCGTGTGCTT |