From: Interleukin-6 increases inner cell mass numbers in bovine embryos
Study | Treatment | Treatment date(s) | IVF replicates | Blastocysts examined | ICM number | TE number | Total number | ICM:TE Ratio |
---|---|---|---|---|---|---|---|---|
A | 0 ng/ml IL6 | Day 5 | 4 | 39 | 53.9 ± 4.2a | 113.5 ± 5.1a | 167.4 ± 8.3a | 0.47 ± 0.03a |
1 ng/ml IL6 | Day 5 | 35 | 52.2 ± 3.3a | 108.6 ± 6.3a | 160.8 ± 8.0a | 0.51 ± 0.04a | ||
10 ng/ml IL6 | Day 5 | 34 | 60 ± 3.6a | 116.2 ± 6.5a | 176.2 ± 8.2a | 0.56 ± 0.05a | ||
100 ng/ml IL6 | Day 5 | 34 | 98 ± 6.5b | 113.2 ± 6.3a | 211.2 ± 10.3b | 0.92 ± 0.07b | ||
B | 0 ng/ml IL6 | Day 3 | 3 | 16 | 36.8 ± 4.3a | 95.6 ± 8.1a | 132.5 ± 11.7a | 0.38 ± 0.03a |
100 ng/ml IL6 | Day 3 | 11 | 61.8 ± 8.1b | 106.3 ± 10.6a | 168.1 ± 14.0a†| 0.65 ± 0.11b | ||
100 ng/ml IL6 | Day 5 | 11 | 58.9 ± 7.6b | 97 ± 16.5a | 155.9 ± 22.5a | 0.7 ± 0.09b | ||
100 + 100 ng/ml IL6 | Day 3 + 5 | 12 | 74.3 ± 8.7b | 87.3 ± 7.2a | 161.5 ± 13.4a | 0.87 ± 0.10c | ||
C | 0 ng/ml IL6 | Day 1 | 4 | 27 | 56.4 ± 4.1a | 111 ± 7.9a | 167.3 ± 9.8a | 0.56 ± 0.05ab |
1 ng/ml IL6 | Day 1 | 39 | 52.5 ± 3.0a | 111.7 ± 5.4a | 164.2 ± 7.3a | 0.49 ± 0.03a | ||
10 ng/ml IL6 | Day 1 | 39 | 59.3 ± 3.9a | 102.3 ± 4.7a | 161.6 ± 6.8a | 0.62 ± 0.05b | ||
100 ng/ml IL6 | Day 1 | 41 | 88.3 ± 4.6b | 112.7 ± 5.0a | 200.9 ± 7.6b | 0.82 ± 0.05c | ||
D | 0 ng/ml IL6 | Day 1 | 5 | 28 | 46.4 ± 3.6a | 99.6 ± 7.2a | 146 ± 8.6a | 0.52 ± 0.05a |
100 ng/ml IL6 | Day 1 | 27 | 79.2 ± 4.7b | 112.1 ± 7.0a | 191.3 ± 10.2b | 0.75 ± 0.05b | ||
100 ng/ml IL6 | Day 5 | 28 | 79.4 ± 6.0b | 108.1 ± 6.6a | 187.5 ± 10.6b | 0.77 ± 0.05b | ||
100 + 100 ng/ml IL6 | Day 1 + 5 | 28 | 100 ± 8.4c | 106.2 ± 4.8a | 206.2 ± 10.1b | 0.98 ± 0.09c | ||
200 ng/ml IL6 | Day 1 | 27 | 77.4 ± 6.9b | 104.5 ± 5.9a | 181.9 ± 11.3b | 0.75 ± 0.06b | ||
E | 0 ng/ml IL6 | Day 5 | 4 | 41 | 56.4 ± 4.1a | 112.3 ± 7.5a | 168 ± 10.3a | 0.53 ± 0.03a |
100 ng/ml IL6 | Day 5 |  | 38 | 88.7 ± 6.2b | 111.4 ± 6.9a | 200.1 ± 8.4b | 0.83 ± 0.05b | |
200 ng/ml IL6 | Day 5 |  | 44 | 93.7 ± 5.2b | 116.6 ± 5a | 210.3 ± 8.4b | 0.83 ± 0.05b | |
100 + 100 ng/ml IL6 | Day 1 + 5 |  | 44 | 89.8 ± 5.7b | 115 ± 6.5a | 204.8 ± 10.5b | 0.81 ± 0.05b |