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Fig. 3 | BMC Developmental Biology

Fig. 3

From: Drosophila ML-DmD17-c3 cells respond robustly to Dpp and exhibit complex transcriptional feedback on BMP signaling components

Fig. 3

Transcription of pathway components is auto-regulated in Dpp-treated ML-DmD17-c3 cells. Relative expression of the indicated genes (a-h), normalized to Act5C expression, was measured in untreated cells (white bars) and in those treated with 1 nM, 3 nM or 5 nM recombinant Dpp (light gray, medium gray, and black, respectively). The Y-axes are scaled to the maximum expression for each gene. Each bar represents the mean and standard deviation of a single assay, consisting of 2–3 technical replicates. Baseline gene expression in untreated cells was normalized to 1 within each trial and the order of trials is maintained across treatments; “Trial 3” for 1 nM Dpp was not completed. Data were analyzed using a general linear model in SPSS (IBM) and “Trial” was treated as a random factor. A posthoc Bonferroni test was used to calculate pairwise P values; * P < 0.05, ** P < 0.01, *** P < 0.001

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