Fig. 2

Put and Tkv are the primary transducers of Dpp signaling in ML-DmD17-c3 cells. Relative Dad (a, c) and brk (b, d) expression, normalized to Act5C expression, was measured in D17 cells treated with control (gfp) or receptor (tkv, sax, put, wit) RNAi, as indicated, in the presence (a, b; filled bars) and absence (c, d; open bars) of 2.5 nM recombinant Dpp. Values given represent the mean and standard deviation of two independent assays, each with 2–3 technical replicates. Data were analyzed using a general linear model in SPSS (IBM) and “Trial” was treated as a random factor. A posthoc Bonferroni test was used to calculate pairwise P values; * P < 0.05, ** P < 0.01, *** P < 0.001