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Fig. 5 | BMC Developmental Biology

Fig. 5

From: Histone acetyltransferase CBP-related H3K23 acetylation contributes to courtship learning in Drosophila

Fig. 5

Inhibition of CBP in the H3K23A overexpressing strain did not aggravate the learning defect. 3μM ICG-001 or equal volume DMSO were mixed into the food. The flies were raised in the medium with either ICG-001 ow DMSO. a After treatment with ICG-001, the H3K23ac level in the larval brains were detected by western blotting. The data was measured by the software Image J. b The expression levels of neuronal genes were examined by RT-qPCR in the larval brains followed the treatment with ICG-001 in the elav-GAL4 strain. The mRNA levels of neuronal genes were normalized to the levels of rp49. Unpaired t-test with Welch’s correction was used. Error bars represent the standard error of the mean. N=3 biological replicates. c Courtship learning analyses of flies. Learning indexes were calculated after treatment with ICG-001. Unpaired t-test was used for statistics. Error bars represent the standard error of the mean; the number of samples was indicated in the bar. n.s., not significant. *p<0.05, **p<0.01

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