Fig. 4

Impaired learning ability in dCBP RNAi Drosophila may due to decreased neuronal gene expression, which is regulated by the level of H3K23ac. a-b The expression levels of neuronal genes were examined by RT-qPCR in the larval brains of dCBP RNAi. The mRNA levels of neuronal genes were normalized to the levels of rp49. Unpaired t-test with Welch’s correction was used. N=3 biological replicates. c The ChIP assay and RT-qPCR analysis validated the decreased enrichment of H3K23ac in the neuronal genes after the silencing of dCBP in the larval brains. The enrichment of H3K23ac was normalized to input. The intergenic region was the euchromatic control region. Error bars represent the standard error of the mean. (N=2 biological replicates). n.s., not significant, *p<0.05, **p<0.01, ***p<0.001, #p<0.05 (elav-GAL4 group compared to H3WT group)