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Table 1 Antisera utilized for immunofluorescence and immunoblot analyses

From: Fermitin family homolog-2 (FERMT2) is highly expressed in human placental villi and modulates trophoblast invasion

Antisera

Method/Dilution Used

Company

Catalogue #

Mouse anti-FERMT2 Clone 3A3

IF: 1:50

IB: 1:4000

EMD Millipore, Etobicoke, ON, CA

MAB2617

Rabbit anti-ITGA5

IF: 1:250

EMD Millipore

AB1928

Rabbit anti-ITGA6

IF: 1:100

Sigma Aldrich, Oakville, ON, CA

HPA012696

Rabbit anti-VWF

IF: 1:200

EMD Millipore

AB7356

Rabbit anti-CDH1

IF: 1:50

Sigma Aldrich

HPA004812

Mouse anti-FERMT1 Clone KN-4

IB: 1:2000

Sigma Aldrich

SAB4200465

Mouse anti-TUBA Clone DM1A

IB: 1:20000

Sigma Aldrich

T6199

Sheep anti-Rabbit FITC

IF: 1:250

Sigma Aldrich

F7512

Donkey anti-Mouse RRX

IF: 1:150

Jackson ImmunoResearch, West Grove, PA, US

715–295-150

ChromPure Mouse IgG

IF: **

Jackson ImmunoResearch

015–000-003

ChromPure Rabbit IgG

IF: **

Jackson ImmunoResearch

011–000-003

Goat anti-Mouse HRP

IB: 1:10000

Promega, Madison, WI, USA

W4021

  1. IF: Immunofluorescence, IB: Immunoblot, FITC: Fluorescein isothiocyanate, RRX: Rhodamine-Red-X, HRP: horseradish peroxidase. **Matched to concentration of primary antisera utilized