Fig. 6

Confirmation of FERMT2 depletion after FERMT2-specific siRNA targeting in HTR8-SVneo cells. Following transfection of HTR8-SVneo cells with FERMT2-specific siRNAs (siF2) or non-targeting siRNAs (non-T), cell lysates were collected from 1 to 4 days post-transfection and targeted protein depletion established by immunoblot analysis. Blots were probed with FERMT1 (F1), FERMT2 (F2) or Tubulin (TUBA)–specific antisera. Tubulin expression served as a loading control. Representative immunoblots are shown. For densitometric analysis, the relative expression of F1 and F2 was calculated by normalizing values for FERMT2-depleted cells to corresponding non-T controls. Any differences in sample loading were equalized using corresponding TUBA densitometric values. The densitometric analysis shown is from four independent experiments. F2 expression was suppressed upon siF2 treatment (t-test, *p < 0.002). The expression of F1 was not significantly affected by F2 siRNA treatment confirming the specificity of the siRNAs