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Fig. 6 | BMC Developmental Biology

Fig. 6

From: Contemporaneous effects of diabetes mellitus and hypothyroidism on spermatogenesis and immunolocalization of Claudin-11 inside the seminiferous tubules of mice

Fig. 6

Representative digital images of histogram profile shows DAB brown staining, color pixel intensity for Claudin-11 in the testes of adult mice, exposed to experimental diabetes mellitus and hypothyroidism. (A) From top to bottom rows; panels shows the digital image masks stained with hematoxylin, DAB and threshold respectively. (B) Claudin-11 is expressed in a limited quantity at tight junction in between Sertoli cells of all treated groups that is why analyzed through score calculation. Data are representing mean ± SEM (n = 6) and different labels indicated significant differences among groups at P < 0.05

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