Fig. 1

Generation and characterisation of the Tg(hsp70:HA-dmrt2a) line. a Transgenic construct design. b Heat-shock protocol: embryos were raised at 28 °C and the heat-shock performed for 30 min at 39 °C. c qPCR analysis showing that dmrt2a transcripts are present from bud-stage until 20-somite stage. d Southern blot hybridisation using a ³²P-labelled γ-crystallin probe showing F0 fish with multiple bands (C⁺, positive control), wildtype fish (C⁻, negative control), and F1 generation fish with only one copy of the transgene or multiple copies. e The HA-Dmrt2a protein is produced with the correct molecular weight (57 kDa). f The fusion protein HA-Dmrt2a (red) co-localises with the nucleus (blue). h: hours, min: minutes, ss: somite stage. Scale bar: 30 μm