Table 1 Primers
From: Swimming exercise to control precocious maturation in male seabass (Dicentrarchus labrax)
Gene | FW/RV | Sequence (5′ > 3′) | Length | Tm | GC% | Self complementarity | Self 3′ complementarity | Product length | AC | Authors |
|---|---|---|---|---|---|---|---|---|---|---|
igf1 | Forward primer | GGCTTGGCTAATCTAACTGGCTTC | 24 | 61.75 | 50.00 | 4.00 | 0.00 | 160 | GQ924783.1 | Crespo et al. 2013 |
Reverse primer | TCGCTCAGGGGTTTCATCACTAC | 23 | 62.24 | 52.17 | 3.00 | 0.00 | ||||
amh | Forward primer | TGCAGAGCAAAGCCTGAAAG | 20 | 59.04 | 50.00 | 4.00 | 1.00 | 126 | AM232701.1 | Diaz et Piferrer, 2015 |
Reverse primer | TCAACGGGGAACAAAGACAA | 20 | 57.58 | 45.00 | 2.00 | 0.00 | ||||
fshr | Forward primer | ACTCCACCTCCATCATCTGC | 20 | 59.16 | 55.00 | 3.00 | 2.00 | 177 | AY642113.1 | Rocha et al. 2007 |
Reverse primer | AACGGGGAACAGTCAGTTTG | 20 | 58.32 | 50.00 | 5.00 | 1.00 | ||||
bmp15 | Forward primer | GGCAGATTTGATGGGTCATT | 20 | 56.05 | 45.00 | 4.00 | 1.00 | 117 | AM933668.1 | Halm et al. 2008 |
Reverse primer | CTTTAACAGGAACGGCGAAG | 20 | 57.12 | 50.00 | 4.00 | 0.00 | ||||
erβ2 | Forward primer | GAGCTGGAGAGCAGGAACAA | 20 | 60 | 55.00 | 4.00 | 0.00 | 61 | AJ489524.1 | Designed, partial sequence from Halms et al. 2007 |
Reverse primer | AGACCAGAGCATCGATCACC | 20 | 60 | 55.00 | 6.00 | 2.00 | ||||
gsdf1 | Forward primer | ACAGAGCTGCCTTGCAATCC | 20 | 60.96 | 55.00 | 6.00 | 4.00 | 99 | JQ755271.1 | Crespo et al. 2013 |
Reverse primer | TCTTGTATGACAAAGCCTGCC | 21 | 58.56 | 47.62 | 6.00 | 1.00 | ||||
arα | Forward primer | CCCCGGATCTTGTGTTCA | 18 | 60 | 55.56 | 4.00 | 2.00 | 70 | AY647256.1 | Designed, comlete sequence from Blazquez et Piferrer, 2015 |
Reverse primer | TTCATCCGTATGCAGTGTTCA | 21 | 60 | 42.86 | 6.00 | 2.00 | ||||
3βhsd | Forward primer | CACCCTACAAGAGCTACGAGGA | 22 | 60 | 54.55 | 4.00 | 0.00 | 96 | JQ861952.1 | Designed, partial sequence from Mazon et Gomez, 2012 |
Reverse primer | CCAGAACCCAGAGGACCAG | 19 | 60 | 63.16 | 3.00 | 1.00 | ||||
11βhsd | Forward primer | GGAAATGCTGGCAACCAC | 18 | 60 | 55.56 | 4.00 | 2.00 | 65 | AF449173.2 | Designed, complete sequence from Mylonas et al. 2007 |
Reverse primer | CACGAACACGGAGCAACA | 18 | 60 | 55.56 | 2.00 | 0.00 | ||||
smc1β | Forward primer | TTCAGACCGATGGACAACCT | 20 | 60 | 50.00 | 3.00 | 0.00 | 87 | KF699104.1 | This study |
Reverse primer | GGCGGGTTTGTAACTGTGAA | 20 | 60 | 50.00 | 3.00 | 1.00 | ||||
inhba | Forward primer | TCATCAAGAAGGACATCCAGAA | 22 | 58.62 | 40.91 | 4.00 | 1.00 | 62 | HE967317.1 | Designed, complete sequence from Garcia-Lopez et al. 2012 |
Reverse primer | GGTTCGGTGTTTACGAGCAG | 20 | 59.21 | 55.00 | 3.00 | 1.00 | ||||
l17 | Forward primer | CTGGCTTGCCTTTCTTGACT | 20 | 60 | 50.00 | 4.00 | 1.00 | 201 | AF139590 | Versamos et al. 2006 |
Reverse primer | GAGGACGTGGTGGTTCATCT | 21 | 60 | 55.00 | 4.00 | 0.00 |